Methods of investigating the effectiveness of anticancer cytotoxic drugs and biologic inhibitors. These include in vitro cell-kill models and cytostatic dye exclusion tests as well as in vivo measurement of tumor growth parameters in laboratory animals.
Archaeal enzymes of the DNA TOPOISOMERASES TYPE I class that catalyze ATP-independent breakage of one of the two strands of DNA, passage of the unbroken strand through the break, and rejoining of the broken strand. These archaeal enzymes reduce the topological stress in the DNA structure by relaxing negatively, but not positively, supercoiled DNA.
A group of diseases related to a deficiency of the enzyme ARGININOSUCCINATE SYNTHASE which causes an elevation of serum levels of CITRULLINE. In neonates, clinical manifestations include lethargy, hypotonia, and SEIZURES. Milder forms also occur. Childhood and adult forms may present with recurrent episodes of intermittent weakness, lethargy, ATAXIA, behavioral changes, and DYSARTHRIA. (From Menkes, Textbook of Child Neurology, 5th ed, p49)
A group of diseases related to a deficiency of the enzyme ARGININOSUCCINATE SYNTHASE which causes an elevation of serum levels of CITRULLINE. In neonates, clinical manifestations include lethargy, hypotonia, and SEIZURES. Milder forms also occur. Childhood and adult forms may present with recurrent episodes of intermittent weakness, lethargy, ATAXIA, behavioral changes, and DYSARTHRIA. (From Menkes, Textbook of Child Neurology, 5th ed, p49)
A species of ARENAVIRUS, part of the New World Arenaviruses (ARENAVIRUSES, NEW WORLD), causing Argentinian hemorrhagic fever. The disease is characterized by congestion, edema, generalized lymphadenopathy and hemorrhagic necrosis and is sometimes fatal.
Conditions which affect the arteries of the brain, meninges, and intracranial portions of the cranial nerves. Relatively common disease processes in this category include: ATHEROSCLEROSIS; emboli; thrombosis (INTRACRANIAL EMBOLISM AND THROMBOSIS); VASCULITIS, CENTRAL NERVOUS SYSTEM; CEREBROVASCULAR TRAUMA; vasospasm (VASOSPASM, INTRACRANIAL); INTRACRANIAL ARTERIOVENOUS MALFORMATIONS; and infections.
DNA constructs that are composed of, at least, a REPLICATION ORIGIN, for successful replication, propagation to and maintainance as an extra chromosome in bacteria. In addition, they can carry large amounts (about 200 kilobases) of other sequence for a variety of bioengineering purposes.
Chromosomes in which fragments of exogenous DNA ranging in length up to several hundred kilobase pairs have been cloned into yeast through ligation to vector sequences. These artificial chromosomes are used extensively in molecular biology for the construction of comprehensive genomic libraries of higher organisms.
DNA constructs that are derived from the DNA of P1 BACTERIOPHAGE. They can carry large amounts (about 100-300 kilobases) of other sequence for a variety of bioengineering purposes.
DNA constructs that are composed of, at least, all elements, such as a REPLICATION ORIGIN; TELOMERE; and CENTROMERE, that are required for successful replication, propagation to and maintainance in progeny mammalian cells. In addition, they are constructed to carry other sequences for analysis or gene transfer.