RIPA Definition
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RIPA: Sensitive assay using radiolabeled antigens to detect specific antibodies in serum. The antigens are allowed to react with the serum and then precipitated using a special reagent such as protein A sepharose beads. The bound radiolabeled immunoprecipitate is then commonly analyzed by gel electrophoresis. Radioimmunoprecipitation assay (RIPA) is often used as a confirmatory test for diagnosing the presence of HIV antibodies.
Synonym: radioimmunoprecipitation assay, radioimmunoprecipitation analysis, radioimmune precipitin assay, farr assay.