Hemolytic Titration Assay Definition

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Hemolytic Titration Assay: Usual screening assay for complement. Dilutions of the serum to be tested are added to antibody-coated erythrocytes and the percentage of lysis is measured. The values are expressed by CH50, hemolytic complement units per milliliter, which is the dilution of serum required to lyse 50 percent of the erythrocytes in the assay.

Synonym: ch50 assay, total hemolytic complement, complement hemolytic activity assay, complement h50, ch(50), whole complement titer, serum complement titer.

Additional tags: hemolytic titration